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96-well, luminometer-compatible tissue-culture plates  (Greiner Bio)


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    Greiner Bio 96-well, luminometer-compatible tissue-culture plates
    96 Well, Luminometer Compatible Tissue Culture Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96-well+luminometer-compatible+tissue+culture+plates/96+well+luminometer+plates/pm40424778-618-13-16
    Average 90 stars, based on 1 article reviews
    96-well, luminometer-compatible tissue-culture plates - by Bioz Stars, 2026-09
    90/100 stars

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    Spectrophotometry:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Microscopy:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Affinity Column:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Luciferase:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Lysis:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    CCK-8 Assay:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Cytotoxicity Assay:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    SPR Assay:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Software:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Analogues:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.

    Infection:

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, Westborough, MA, USA); Protein Preparation Wizard implemented with Maestro (Schrödinger Maestro Version 11.5.011, New York, NY, USA, mM share Version 4.1.011, New York, NY, USA, Release 2018-1, Platform Darwin-x86_64).

    Article Title: Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability
    Article Snippet: U87.CD4.CCR5 cells were seeded in Greiner Bio-one 96-well luminometer-compatible tissue culture plates with a density of 1.2× 104 cells/well.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, ountain View, CA, USA); calcium ph sphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madiso , WI, USA); mouse anti-p24 (ab9071, bcam, Cambrid e, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, O, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue cult re lates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 2.02 ± 0.81 0.73 ± 0.56 0.45 ± 0.35 0.45 ± 0.33 1.15 ± 0.81 0.96 ± 0.59 73 ± 14 75.85 SC56 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.43 ± 0.1 0.44 ± 0.17 2.02 ± 0.25 NA 1.35 ± 0.44 1.06 ± 0.668 9.2 ± 1.1 8.65 SC55 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Discovery of phenylalanine derivatives as potent HIV-1 capsid inhibitors from click chemistry-based compound library
    Article Snippet: Briefly, U87.CD4.CCR5 (1.2 × 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner bio-one).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmington, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate luminometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 7.1 ± 4.2 6.9 ± 5.2 28.92 ± 5.62 24.28 ± 6.2 22.6 ± 4.2 18 ± 9 120 ± 18 6.63 S 52 Molecules 2018, 23, x 9 of 30 Table 4.

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: Briefly, U87.CD4.CCR5/CXCR4 (1.2× 104 cells/well) target cells were seeded in 96-well luminometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA).

    Article Title: Field-Based Affinity Optimization of a Novel Azabicyclohexane Scaffold HIV-1 Entry Inhibitor
    Article Snippet: General Information DMEM, FBS, penicillin, streptomycin and L-glutamine, G418, puromycin, MultiskanTM GO Microplate Spectrophotometer, FEI Talos Arctica electron microscope, FEI Ceta 16M CMOS camera (Thermo Scientific, Waltham, MA, USA); BL21-Codon Plus (DE3)-RIL Competent Cells (Agilent Technologies, Wilmi gton, DE, USA); Talon cobalt resin affinity column (Clonetech Laboratories, Mountain View, CA, USA); calcium phosphate, 5X luciferase lysis buffer, luciferase assay substrate, GloMax 96 microplate lu inometer (Promega, Madison, WI, USA); mouse anti-p24 (ab9071, Abcam, Cambridge, MA, USA); Triton X-100, O-phenylenediamine, phosphate-citrate buffer with sodium perborate, DMSO (Sigma-Aldrich, St. Louis, MO, USA); 96-well lu inometer-compatible tissue culture plates (Greiner Bio-one, Monroe, NC, USA); Cell Counting Kit-8 cell proliferation and cytotoxicity assay (Dojindo Molecular Technologies, Rockville, MD, USA); 96-well tissue culture plates (Olympus Plastics, San Diego, CA, USA); ProteOn XPR36 SPR Protein Interaction Array System, GLH sensorchip, (Bio-Rad Laboratories, Hercules, CA, USA); MicroCal VP-Capillary differential scanning calorimeter, Automated Origin 7.0 software (Malvern Panalytical, 0.051 ± 0.008 0.011 ± 0.003 0.046 ± 0.009 0.13 ± 0.015 0.11 ± 0.049 0.0698 ± 0.045 94 ± 10 1344.71 As summarized in Table 4, SC28 and each of the analogues tested exhibited antiviral effects in the single-round infection assay utilizing viruses pseudotyped with HIV-1 Env from either the B41, HxBc2, JR-CSF, JR-FL, r YU-2 isolates.



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